The southeastern myotis is distinguishable from other Myotis species by its woolly, bi-colored fur (dark brown at the base with pale tips) and especially long toe hairs. Summer habitat includes hardwood and mixed forests, coastal areas, and a wide range of riparian habitat. Maternity colonies are often associated with caves, but have also been observed in hollow trees and man-made structures like bridges and culverts. In its southern range, the species remains active for much of the winter, but individuals in the northern range hibernate in some of the same structures used for summer roosts (e.g., caves). Females of this species typically give birth to twin pups. Diet consists primarily of mosquitoes, crane flies, and moths.